Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus)

Sumba Ongole (SO) cattle are from India which were imported to Indonesia during the Dutch colonial era and well adapted on Island, East Nusa Tenggara, Indonesia. known for high meat production. This research was conducted identify one point mutation of g.415C/A (F94L marker) in myostatin (MSTN) gene (exon 1) using PCR-RFLP method with TaqI restriction enzyme (T*CGA). DNA samples SO bulls cows (Total 153) Island used this study. Results revealed that all study had CC genotype C allele as common allele. The not found However, g.400G/A detected without amino acid changes (synonymous). It concluded MSTN/TaqI present is monomorphic cannot be genetic marker productive traits cattle.